Measurements of protein-protein interactions by size exclusion chromatography

被引：46

作者：

Bloustine, J ^{[1
]}

Berejnov, V ^{[1
]}

Fraden, S ^{[1
]}

机构：

[1] Brandeis Univ, Martin Fisher Sch Phys, Complex Fluids Grp, Waltham, MA 02454 USA

来源：

BIOPHYSICAL JOURNAL | 2003年 / 85卷 / 04期

基金：

美国国家航空航天局;

关键词：

D O I：

10.1016/S0006-3495(03)74684-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A method is presented for determining second virial coefficients (B-2) of protein solutions from retention time measurements in size exclusion chromatography. We determine B-2 by analyzing the concentration dependence of the chromatographic partition coefficient. We show the ability of this method to track the evolution of B-2 from positive to negative values in lysozyme and bovine serum albumin solutions. Our size exclusion chromatography results agree quantitatively with data obtained by light scattering.

引用

页码：2619 / 2623

页数：5

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