Cloning, sequencing, and expression of a gene from Campylobacter jejuni encoding a protein (Omp18) with similarity to peptidoglycan-associated lipoproteins

被引：16

作者：

Konkel, ME ^{[1
]}

Mead, DJ ^{[1
]}

Cieplak, W ^{[1
]}

机构：

[1] NIAID,INTRACELLULAR PARASITES LAB,ROCKY MT LABS,HAMILTON,MT 59840

来源：

INFECTION AND IMMUNITY | 1996年 / 64卷 / 05期

关键词：

D O I：

10.1128/IAI.64.5.1850-1853.1996

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A Campylobacter jejuni genomic plasmid library was screened with antiserum generated against whole C. jejuni, revealing two immunoreactive clones. Sequence analysis of the recombinant plasmids revealed a common open reading frame of 498 nucleotides encoding a protein of 165 amino acids with a calculated molecular mass of 18,018 Da. The recombinant product partitioned to the outer membrane fractions of Escherichia coli transformants and has been designated Omp18. The deduced amino acid sequence of the cloned C. jejuni gene exhibits considerable similarity to peptidoglycan-associated lipoproteins from other gram-negative bacteria.

引用

页码：1850 / 1853

页数：4

共 27 条

[21]

PEI ZH, 1991, J BIOL CHEM, V266, P16363

[22] THE COMPLETE GENERAL SECRETORY PATHWAY IN GRAM-NEGATIVE BACTERIA [J].

PUGSLEY, AP .

MICROBIOLOGICAL REVIEWS, 1993, 57 (01) :50-108

[23]

SAMBROOK J, 1989, MOL CLONING LAB MANU, P75

[24]

SANKARAN K, 1995, METHOD ENZYMOL, V250, P683

[25] MOLECULAR-CLONING, NUCLEOTIDE-SEQUENCE, AND OCCURRENCE OF A 16.5-KILODALTON OUTER-MEMBRANE PROTEIN OF BRUCELLA-ABORTUS WITH SIMILARITY TO PAL LIPOPROTEINS [J].

TIBOR, A ;

WEYNANTS, V ;

DENOEL, P ;

LICHTFOUSE, B ;

DEBOLLE, X ;

SAMAN, E ;

LIMET, JN ;

LETESSON, JJ .

INFECTION AND IMMUNITY, 1994, 62 (09) :3633-3639

[26] ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS [J].

TOWBIN, H ;

STAEHELIN, T ;

GORDON, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (09) :4350-4354

[27]

WU HC, 1986, CURR TOP MICROBIOL, V125, P127

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