Genetic analysis of the gene cluster for the synthesis of serotype a-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans

被引：25

作者：

Suzuki, N ^{[1
]}

Nakano, Y ^{[1
]}

Yoshida, Y ^{[1
]}

Nakao, H ^{[1
]}

Yamashita, Y ^{[1
]}

Koga, T ^{[1
]}

机构：

[1] Kyushu Univ, Fac Dent Sci, Dept Prevent Dent, Fukuoka 8128582, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2000年 / 1517卷 / 01期

关键词：

serotype antigen; polysaccharide; Actinobacillus actinomycetemcomitans;

D O I：

10.1016/S0167-4781(00)00229-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The serotype a-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans consists of 6-deoxy-D-talose. A gene cluster associated with the biosynthesis of SPA was cloned and sequenced from the chromosomal DNA of A. actinomycetemcomitans SUNYaB 75 (serotype a). This cluster consisted of 14 open reading frames. Insertional inactivation of eight genes in this cluster resulted in loss of the ability of A. actinomycetemcomitans SUNYaB 75 cells to produce the polysaccharide. A protein database search revealed that the 11 sequential genes containing these eight genes were not found in SPA-associated gene clusters of the other serotypes of A. actinomycetemcomitans. These results suggest that the gene cluster is unique to serotype a and is essential to the synthesis of the SPA. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：135 / 138

页数：4

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