Activity regulation and physiological impacts of maize C4-specific phosphoenolpyruvate carboxylase overproduced in transgenic rice plants

被引:81
作者
Fukayama, H
Hatch, MD
Tamai, T
Tsuchida, H
Sudoh, S
Furbank, RT
Miyao, M [1 ]
机构
[1] Natl Inst Agrobiol Sci, Photosynth Lab, Tsukuba, Ibaraki 3058602, Japan
[2] CSIRO Plant Ind, Canberra, ACT 2601, Australia
关键词
C(4) photosynthesis; phosphoenolpyruvate carboxylase; protein phosphorylation; respiration; transgenic rice;
D O I
10.1023/A:1025861431886
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Phosphoenolpyruvate carboxylase ( PEPC) was overproduced in the leaves of rice plants by introducing the intact maize C(4)-specific PEPC gene. Maize PEPC in transgenic rice leaves underwent activity regulation through protein phosphorylation in a manner similar to endogenous rice PEPC but contrary to that occurring in maize leaves, being downregulated in the light and upregulated in the dark. Compared with untransformed rice, the level of the substrate for PEPC ( phosphoenolpyruvate) was slightly lower and the product ( oxaloacetate) was slightly higher in transgenic rice, suggesting that maize PEPC was functioning even though it remained dephosphorylated and less active in the light. (14)CO(2) labeling experiments indicated that maize PEPC did not contribute significantly to the photosynthetic CO(2) fixation of transgenic rice plants. Rather, it slightly lowered the CO(2) assimilation rate. This effect was ascribable to the stimulation of respiration in the light, which was more marked at lower O(2) concentrations. It was concluded that overproduction of PEPC does not directly affect photosynthesis significantly but it suppresses photosynthesis indirectly by stimulating respiration in the light. We also found that while the steady-state stomatal aperture remained unaffected over a wide range of humidity, the stomatal opening under non-steady-state conditions was destabilized in transgenic rice.
引用
收藏
页码:227 / 239
页数:13
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