Chimeric anti-tenascin antibody 81C6: Increased tumor localization compared with its murine parent

被引：21

作者：

Zalutsky, MR ^{[1
]}

Archer, GE ^{[1
]}

Garg, PK ^{[1
]}

Batra, SK ^{[1
]}

Bigner, DD ^{[1
]}

机构：

[1] DUKE UNIV,MED CTR,DEPT PATHOL,DURHAM,NC 27710

来源：

NUCLEAR MEDICINE AND BIOLOGY | 1996年 / 23卷 / 04期

关键词：

monoclonal antibody; chimeric antibody; radioiodination; glioma;

D O I：

10.1016/0969-8051(96)00021-2

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

When labeled using the Iodogen method, a chimeric antibody composed of the human IgG, constant region and the variable regions of murine anti-tenascin 81C6 exhibited superior uptake in human glioma xenografts compared with its murine parent. In the current study, three paired label experiments were performed in athymic mice with subcutaneous D-54 MG human glioma xenografts to evaluate further the properties of radioiodinated chimeric 81C6. These studies demonstrated that (a) the enhanced tumor uptake of chimeric 81C6 is specific; (b) when labeling was performed using N-succinimidyl 3-iodobenzoate, chimeric 81C6 again showed preferential accumulation in tumor compared with murine 81C6; and (c) the tumor uptake advantage observed previously with murine 81C6 for N-succinimidyl 3-iodobenzoate compared with Iodogen labeling did not occur with chimeric 81C6.

引用

页码：449 / 458

页数：10

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[31] MOUSE HUMAN CHIMERIC ANTIBODIES TO A TUMOR-ASSOCIATED ANTIGEN - BIOLOGIC ACTIVITY OF THE 4 HUMAN-IGG SUBCLASSES [J].