Electron-immunocytochemical localization of P2X1 receptors in the rat cerebellum

被引：88

作者：

Loesch, A

Burnstock, G

机构：

[1] UCL, Dept Anat & Dev Biol, London WC1E 6BT, England

[2] Royal Free Hosp, Sch Med, Auton Neurosci Inst, London NW3 2PF, England

来源：

CELL AND TISSUE RESEARCH | 1998年 / 294卷 / 02期

关键词：

P2X(1) receptor; ultrastructure; cerebellum; rat (Wistar);

D O I：

10.1007/s004410051175

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The distribution of the P2X(1) subtype of purinoceptors associated with the extracellular activities of ATP was studied in the rat cerebellum at the electron-microscope level. Receptors were labelled with peroxidase-antiperoxidase and the avidin-biotin-peroxidase complex for immunocytochemistry. Immunoreactivity to P2X(1) receptors was localized in subpopulations of synapses between varicosities of parallel fibres of granule cells and dendritic spines of Purkinje cells. Unlabelled varicosities of parallel fibres formed asymmetric synapses with labelled dendritic spines, whereas labelled varicosities of parallel fibres formed asymmetric synapses with unlabelled dendritic spines. P2X(1) immunoreactivity was also localized in some astrocyte processes. The functional significance of these findings is discussed.

引用

页码：253 / 260

页数：8

共 47 条

[1] PURINOCEPTORS - ARE THERE FAMILIES OF P2X AND P2Y PURINOCEPTORS [J].