Developmental regulation of invariant chain proteolysis controls MHC class II trafficking in mouse dendritic cells

Dendritic cells (DCs) developmentally regulate their capacity for antigen presentation by controlling the transport and surface expression of MHC class II molecules. These events reflect a developmental regulation of invariant (li) chain cleavage, most likely by the cysteine protease cathepsin S. In immature DCs, inefficient Il chain cleavage due to low cathepsin S activity leads to the transport of class II-ii chain complexes to lysosomes, while in mature DCs, elevated cathepsin S activity results in efficient delivery of class II alpha beta dimers to.the plasma membrane. Cathepsin S is not controlled transcriptionally but by a novel mechanism involving alterations in the expression and localization of an endogenous cathepsin S inhibitor cystatin C. Thus, the ratio of cystatin C to cathepsin S in developing DCs helps to determine the fate of newly synthesized MHC class II molecules.