The minotaur proteome: Avoiding cross-species identifications deriving from bovine serum in cell culture models

被引:40
作者
Bunkenborg, Jakob [2 ]
Espadas Garcia, Guadalupe [1 ]
Pena Paz, Marcia Ivonne [3 ]
Andersen, Jens S. [2 ]
Molina, Henrik [1 ]
机构
[1] CRG, Barcelona 08003, Spain
[2] Univ So Denmark, Dept Biochem & Mol Biol, Odense M, Denmark
[3] Univ Pompeu Fabra, Barcelona, Spain
关键词
Artifacts; Bioinformatics; Cell culture; MS; SILAC; MASS-SPECTROMETRY; ADSORPTION; PROTEINS;
D O I
10.1002/pmic.201000103
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cell culture is a fundamental tool in proteomics where mammalian cells are cultured in vitro using a growth medium often supplemented with 5-15% FBS. Contamination by bovine proteins is difficult to avoid because of adherence to the plastic vessel and the cultured cells. We have generated peptides from bovine serum using four sample preparation methods and analyzed the peptides by high mass accuracy LC-MS/MS. Distinguishing between bovine and human peptides is difficult because of a considerable overlap of identical tryptic peptide sequences. Pitfalls in interpretation, different database search strategies to minimize erroneous identifications and an augmented contaminant database are presented.
引用
收藏
页码:3040 / 3044
页数:5
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