Enzymology of UDP-glucose:sinapic acid glucosyltransferase from Brassica napus

被引：19

作者：

Wang, SX ^{[1
]}

Ellis, BE ^{[1
]}

机构：

[1] Univ British Columbia, Dept Plant Sci, Vancouver, BC V6T 1Z4, Canada

来源：

PHYTOCHEMISTRY | 1998年 / 49卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Brassica napus; Cruciferae; UDP-glucose : sinapic acid glucosyltransferase; sinapine; enzymology;

D O I：

10.1016/S0031-9422(98)00252-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

UDP-glucose:sinapic acid glucosyltransferase (SGT; EC 2.4.1.120) was purified from 60-h-old seedlings of Brassica napus. The purified SGT appears to be a cytosolic monomeric polypeptide with a M-r of 42 kDa and a pI of 5. Kinetic analysis suggested that the catalytic mechanism used by SGT best fits a "random bi-bi" model, with a K m(UDP-glucose) of 2.4 mM and K-m(sinapic acid) of 0.16 mM. SGT also catalyzes the reverse reaction in vitro, using UDP and sinapoylglucose to form UDP-glucose. No cofactors are required for enzyme activity, but reducing agents and glycerol are required to stabilize the activity. The enzyme is strongly inhibited by p-OH-mercuribenzoic acid, UDP, TDP, Zn++, Cu++ and Hg++. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：307 / 318

页数：12

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