A functionalized THz sensor for marker-free DNA analysis

被引：130

作者：

Nagel, M ^{[1
]}

Richter, F ^{[1
]}

Haring-Bolívar, P ^{[1
]}

Kurz, H ^{[1
]}

机构：

[1] Univ Aachen, Inst Semicond Elect, D-52074 Aachen, Germany

来源：

PHYSICS IN MEDICINE AND BIOLOGY | 2003年 / 48卷 / 22期

关键词：

D O I：

10.1088/0031-9155/48/22/001

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We report on a novel resonant THz sensor for the label-free analysis of DNA molecules. The sensor allows the direct detection of DNA-probe molecules at functionalized electrodes via hybridization. Subsequent time resolved photoconductive sampling of the THz transmission identifies the binding state between probe and target DNA. Integrating neighbouring sensors on a chip, this technique can be extended to a parallel analysis of multiple DNA sequences. A clearly readable sensor response is obtained with less then 40 fmol of 20-mer single-stranded DNA molecules, indicating at least a sevenfold sensitivity increase compared to previous approaches.

引用

页码：3625 / 3636

页数：12

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