Microarray analysis of nicotine-induced changes in gene expression in endothelial cells

被引:74
作者
Zhang, SL [1 ]
Day, INM [1 ]
Ye, S [1 ]
机构
[1] Univ Southampton, Sch Med, Human Genet Res Div, Southampton SO16 6YD, Hants, England
关键词
microarray; inositol phospholipid pathway; cAMP response element binding protein; nuclear factor-kappa B;
D O I
10.1152/physiolgenomics.2001.5.4.187
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Cigarette smoking causes vascular endothelial dysfunction and is a major risk factor for cardiovascular diseases. Nicotine, a major constituent of cigarette smoke, has been shown to alter gene expression in endothelial cells; however, the regulatory pathways involved remain to be defined. We hypothesized that there might be distinct pathways that could be identified by systematic transcriptome analysis. Using the cDNA microarray approach, we ascertained the expression of over 4,000 genes in human coronary artery endothelial cells and identified a number of nicotine-modulated genes encoding a protein involving in signal transduction or transcriptional regulation. Among these were phosphatidylinositol phosphate kinase and diacylglycerol kinase, which are regulators of the inositol phospholipid pathway. Changes were also detected for transcription factors cAMP response element binding protein and nuclear factor-kappaB, of which the activities of both have been previously shown to be altered in nicotine-stimulated cells. The data from this study are relevant to understanding the mechanisms underlying the pathophysiological effect of nicotine and smoking, particularly on endothelial function and pathogenesis of atherosclerosis.
引用
收藏
页码:187 / 192
页数:6
相关论文
共 28 条
[1]  
Alberts B., 1994, MOL BIOL CELL, P721
[2]   Nicotine and cotinine stimulate secretion of basic fibroblast growth factor and affect expression of matrix metalloproteinases in cultured human smooth muscle cells [J].
Carty, CS ;
Soloway, PD ;
Kayastha, S ;
Bauer, J ;
Marsan, B ;
Ricotta, JJ ;
Dryjski, M .
JOURNAL OF VASCULAR SURGERY, 1996, 24 (06) :927-934
[3]   Nicotine regulates basic fibroblastic growth factor and transforming growth factorβ1 production in endothelial cells [J].
Cucina, A ;
Corvino, V ;
Sapienza, P ;
Borrelli, V ;
Lucarelli, M ;
Scarpa, S ;
Strom, R ;
Santoro-D'Angelo, L ;
Cavallaro, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 257 (02) :306-312
[4]   Homocysteine signal cascade: production of phospholipids, activation of protein kinase C, and the induction of c-fos and c-myb in smooth muscle cells [J].
Dalton, ML ;
Gadson, PF ;
Wrenn, RW ;
Rosenquist, TH .
FASEB JOURNAL, 1997, 11 (08) :703-711
[5]   FUNCTIONAL-CHARACTERIZATION OF A NONCLASSICAL NICOTINE RECEPTOR-ASSOCIATED WITH INOSITOLPHOSPHOLIPID BREAKDOWN AND MOBILIZATION OF INTRACELLULAR CALCIUM POOLS [J].
GARNIER, M ;
LAMACZ, M ;
TONON, MC ;
VAUDRY, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (24) :11743-11747
[6]   ROLE OF [NA+](I) AND [CA2+](I) IN NICOTINE-INDUCED NOREPINEPHRINE RELEASE FROM BOVINE ADRENAL CHROMAFFIN CELLS [J].
GERBER, SH ;
HAUNSTETTER, A ;
KRUGER, C ;
KAUFMANN, A ;
NOBILING, R ;
HAASS, M .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1995, 269 (03) :C572-C581
[7]   ACETYLCHOLINE INDUCES VOLTAGE-INDEPENDENT INCREASE OF CYTOSOLIC CALCIUM IN MOUSE MYOTUBES [J].
GIOVANELLI, A ;
GRASSI, F ;
MATTEI, E ;
MILEO, AM ;
EUSEBI, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (22) :10069-10073
[8]   SIGNAL TRANSDUCTION IN ATHEROSCLEROSIS - INTEGRATION OF CYTOKINES AND THE EICOSANOID NETWORK [J].
HAJJAR, DP ;
POMERANTZ, KB .
FASEB JOURNAL, 1992, 6 (11) :2933-2941
[9]   HIGHER LEVELS OF NICOTINE IN ARTERIAL THAN IN VENOUS-BLOOD AFTER CIGARETTE-SMOKING [J].
HENNINGFIELD, JE ;
STAPLETON, JM ;
BENOWITZ, NL ;
GRAYSON, RF ;
LONDON, ED .
DRUG AND ALCOHOL DEPENDENCE, 1993, 33 (01) :23-29
[10]   Fluid shear stress-mediated signal transduction: How do endothelial cells transduce mechanical force into biological responses? [J].
Ishida, T ;
Takahashi, M ;
Corson, MA ;
Berk, BC .
ATHEROSCLEROSIS IV: RECENT ADVANCES IN ATHEROSCLEROSIS RESEARCH: THE FOURTH SARATOGA INTERNATIONAL CONFERENCE ON ATHEROSCLEROSIS, 1997, 811 :12-24