Determining the procedure for routine residue monitoring of sulfamethazine in edible animal tissues

A simplified method to determine/identify residual sulfamethazine (SMZ) in edible tissues from cattle, pigs, chickens and sheep by a high-performance liquid chromatography (HPLC) with a photo-diode array detector is presented. The sample preparation was performed by homogenizing with 30% (v/v) ethanol in water followed by an Ultrafree (R) -MC/PL as a centrifugal ultra-filtration unit. For determination/identification of SMZ, a reversed-phase C-4 column and a mobile phase of 15% (v/v) ethanol in water with a photo-diode array detector was used. Average recoveries from spiked SMZ (0.1-1.0 mg kg(-1)) were greater than or equal to 80% with coefficients of variation between 1.3 and 4.3%. The limits of quantitation were calculated to be 0.057-0.060 mg kg(-1). The total time and solvent required for the analysis of one sample were < 40 min and <2 mL of ethanol, respectively. Copyright (C) 2001 John Wiley & Sons, Ltd.