Screening phage-displayed combinatorial peptide libraries

被引:71
作者
Kay, BK
Kasanov, J
Yamabhai, M
机构
[1] Univ Wisconsin, Dept Pharmacol, Madison, WI 53706 USA
[2] Suranaree Univ Technol, Inst Agr Technol, Sch Biotechnol, Nakon Ratchasima 30000, Thailand
关键词
D O I
10.1006/meth.2001.1185
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Among the many techniques available to investigators interested in mapping protein-protein interactions is phage display. With a modest amount of effort, time, and cost, one can select peptide ligands to a wide array of targets from phage-display combinatorial peptide libraries. In this article, protocols and examples are provided to guide scientists who wish to identify peptide ligands to their favorite proteins. (C) 2001 Academic Press.
引用
收藏
页码:240 / 246
页数:7
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