MTERF4 Regulates Translation by Targeting the Methyltransferase NSUN4 to the Mammalian Mitochondrial Ribosome

被引：240

作者：

Camara, Yolanda ^{[2
,4
,5
]}

Asin-Cayuela, Jorge ^{[6
]}

Park, Chan Bae ^{[7
]}

Metodiev, Metodi B. ^{[8
]}

Shi, Yonghong ^{[1
,2
]}

Ruzzenente, Benedetta ^{[8
]}

Kukat, Christian ^{[8
]}

Habermann, Bianca ^{[8
]}

Wibom, Rolf ^{[2
]}

Hultenby, Kjell ^{[2
]}

Franz, Thomas ^{[8
]}

Erdjument-Bromage, Hediye ^{[9
]}

Tempst, Paul ^{[9
]}

Hallberg, B. Martin ^{[3
]}

Gustafsson, Claes M. ^{[1
]}

Larsson, Nils-Goran ^{[2
,8
]}

机构：

[1] Univ Gothenburg, Inst Biomed, S-40530 Gothenburg, Sweden

[2] Karolinska Inst, Dept Lab Med, S-14186 Huddinge, Sweden

[3] Karolinska Inst, Dept Cell & Mol Biol, S-14186 Huddinge, Sweden

[4] Hosp Univ Vall Hebron, Unitat Patol Mitocondrial, Inst Recerca, Barcelona 08035, Spain

[5] Ctr Invest Biomed Red Enfermedades Raras CIBERER, Barcelona, Spain

[6] Univ Gothenburg, Dept Clin Chem, S-40530 Gothenburg, Sweden

[7] Ajou Univ, Inst Med Sci, Sch Med, Suwon 443721, South Korea

[8] Max Planck Inst Biol Ageing, D-50931 Cologne, Germany

[9] Mem Sloan Kettering Canc Ctr, Mol Biol Program, New York, NY 10065 USA

来源：

CELL METABOLISM | 2011年 / 13卷 / 05期

基金：

瑞典研究理事会;

关键词：

TERMINATION FACTOR MTERF; DNA-BINDING PROTEIN; TRANSCRIPTION FACTOR; OXIDATIVE-PHOSPHORYLATION; RNA-POLYMERASE; IN-VIVO; MTDNA; DISORDERS; SUBUNIT; IMMUNOPRECIPITATION;

D O I：

10.1016/j.cmet.2011.04.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Precise control of mitochondria! DNA gene expression is critical for regulation of oxidative phosphorylation capacity in mammals. The MTERF protein family plays a key role in this process, and its members have been implicated in regulation of transcription initiation and site-specific transcription termination. We now demonstrate that a member of this family, MTERF4, directly controls mitochondrial ribosomal biogenesis and translation. MTERF4 forms a stoichiometric complex with the ribosomal RNA methyltransferase NSUN4 and is necessary for recruitment of this factor to the large ribosomal subunit. Loss of MTERF4 leads to defective ribosomal assembly and a drastic reduction in translation. Our results thus show that MTERF4 is an important regulator of translation in mammalian mitochondria.

引用

页码：527 / 539

页数：13

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