Presence/absence, differential expression and sequence polymorphisms between PiAVR2 and PiAVR2-like in Phytophthora infestans determine virulence on R2 plants

被引：112

作者：

Gilroy, Eleanor M. ^{[1
]}

Breen, Susan ^{[1
,2
]}

Whisson, Stephen C. ^{[1
]}

Squires, Julie ^{[1
]}

Hein, Ingo ^{[3
]}

Kaczmarek, Maciej ^{[1
]}

Turnbull, Dionne ^{[1
]}

Boevink, Petra C. ^{[1
]}

Lokossou, Anoma ^{[4
]}

Cano, Liliana M. ^{[5
]}

Morales, Juan ^{[1
,6
]}

Avrova, Anna O. ^{[1
]}

Pritchard, Leighton ^{[1
]}

Randall, Eva ^{[1
]}

Lees, Alison ^{[1
]}

Govers, Francine ^{[7
,8
]}

van West, Pieter ^{[9
]}

Kamoun, Sophien ^{[5
]}

Vleeshouwers, Vivianne G. A. A. ^{[4
]}

Cooke, David E. L. ^{[1
]}

Birch, Paul R. J. ^{[1
,2
]}

机构：

[1] James Hutton Inst, Dundee DD2 5DA, Scotland

[2] Univ Dundee, Coll Life Sci, Div Plant Sci, JHI, Dundee DD2 5DA, Scotland

[3] James Hutton Inst, Genet Programmes, Dundee DD2 5DA, Scotland

[4] Wageningen Univ, Wageningen UR Plant Breeding, Wageningen, Netherlands

[5] John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

[6] Univ Nacl Colombia sede Medellin, Dept Ciencias Agron, Medellin, Colombia

[7] Wageningen Univ, Phytopathol Lab, Wageningen, Netherlands

[8] Wageningen Univ, Ctr BioSyst Genom, Wageningen, Netherlands

[9] Univ Aberdeen, Inst Med Sci, Coll Life Sci & Med, Aberdeen Oomycete Lab, Aberdeen AB25 2ZD, Scotland

来源：

NEW PHYTOLOGIST | 2011年 / 191卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

durable disease resistance; effector-triggered immunity; gene-for-gene; hypersensitive response; potato blight; RXLR EFFECTORS; PATHOGEN PHYTOPHTHORA; DISEASE RESISTANCE; OOMYCETE EFFECTORS; AVIRULENCE GENES; DOWNY MILDEW; POTATO; LOCUS; AVR3A; PROTEIN;

D O I：

10.1111/j.1469-8137.2011.03736.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A detailed molecular understanding of how oomycete plant pathogens evade disease resistance is essential to inform the deployment of durable resistance (R) genes. Map-based cloning, transient expression in planta, pathogen transformation and DNA sequence variation across diverse isolates were used to identify and characterize PiAVR2 from potato late blight pathogen Phytophthora infestans. PiAVR2 is an RXLR-EER effector that is up-regulated during infection, accumulates at the site of haustoria formation, and is recognized inside host cells by potato protein R2. Expression of PiAVR2 in a virulent P. infestans isolate conveys a gain-of-avirulence phenotype, indicating that this is a dominant gene triggering R2-dependent disease resistance. PiAVR2 presence/absence polymorphisms and differential transcription explain virulence on R2 plants. Isolates infecting R2 plants express PiAVR2-like, which evades recognition by R2. PiAVR2 and PiAVR2-like differ in 13 amino acids, eight of which are in the C-terminal effector domain; one or more of these determines recognition by R2. Nevertheless, few polymorphisms were observed within each gene in pathogen isolates, suggesting limited selection pressure for change within PiAVR2 and PiAVR2-like. Our results direct a search for R genes recognizing PiAVR2-like, which, deployed with R2, may exert strong selection pressure against the P. infestans population.

引用

页码：763 / 776

页数：14

共 43 条

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