Osteoblast growth and function in porous poly ε-caprolactone matrices for bone repair:: a preliminary study

被引:198
作者
Ciapetti, G
Ambrosio, L
Savarino, L
Granchi, D
Cenni, E
Baldini, N
Pagani, S
Guizzardi, S
Causa, F
Giunti, A
机构
[1] Ist Ortoped Rizzoli, Lab Fisiopatol Impianti Ortoped, I-40139 Bologna, Italy
[2] Univ Naples Federico II, CNR, Ctr Interdisciplinare Ric Biomat, Ist Tecnol Mat Compositi, I-80125 Naples, Italy
[3] Univ Parma, Dipartimento Med Sperimentale, I-43100 Parma, Italy
[4] Univ Bologna, Dipartimento Sci Anat Umane & Fisiopatol Apparato, Bologna, Italy
关键词
polymers; osteoblasts; poly epsilon-caprolactone; hydroxyapatite; biodegradation;
D O I
10.1016/S0142-9612(03)00263-1
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Current methods for the replacement of skeletal tissue involve the use of autografts, allografts and, recently, synthetic substitutes, which provide a proper amount of material to repair large bone defects. Engineered bone seems a promising approach, but a number of variables have to be set prior to any clinical application. In this study. four different poly caprolactone-based polymers (PCL) were prepared and tested in vitro using osteoblast-like Saos-2 cells. Differences among three-dimensional polymers include porosity, addition of hydroxyapatite (HA) particles, and treatment with simulated body fluid. Biochemical parameters to assess cell/material interactions include viability, growth, alkaline phosphatase release, and mineralization of osteoblastic cells seeded onto three-dimensional samples, while their morphology was observed using light microscopy and SEM. Preliminary results show that the polymers, though degrading in the medium, have a positive interaction with cells, as they support cell growth and functions. In the short-term culture (3-7 days) of Saos-2 on polymers, little differences were found among PCL samples, with the presence of HA moderately improving the number of cells onto the surfaces. In the long term (3-4 weeks), it was found that the HA-added polymers obtained the best colonization by cells, and more mineral formation was observed after coating with SBF It can be concluded that PCL is a promising material for three-dimensional scaffold for bone formation. and the presence of bone-like components improves osteoblast activity. (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:3815 / 3824
页数:10
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