Total Chemical Synthesis of Di-ubiquitin Chains

被引：151

作者：

Kumar, K. S. Ajish ^{[1
]}

Spasser, Liat ^{[1
]}

Erlich, Lesly A. ^{[1
]}

Bavikar, Sudhir N. ^{[1
]}

Brik, Ashraf ^{[1
]}

机构：

[1] Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 48期

关键词：

ligation; peptides; proteins; synthetic methods; ubiquitin; PEPTIDE UBIQUITYLATION; POLYUBIQUITIN CHAINS; CRYSTAL-STRUCTURE; STRUCTURAL BASIS; ISOPEPTIDASE-T; LIGATION; PROTEINS; BINDING; DESULFURIZATION; EFFICIENT;

D O I：

10.1002/anie.201003763

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Building the chains: A general and effective approach for the total chemical synthesis of all the di-ubiquitin chains (see picture) is presented. The behavior of the synthetic chains in the presence of isopeptidase T, an enzyme responsible for the disassembly of the majority of unanchored polyubiquitin in vivo, was examined. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：9126 / 9131

页数：6

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