An efficient Fmoc-SPPS approach for the generation of thioester peptide precursors for use in native chemical ligation

被引：401

作者：

Blanco-Canosa, Juan B.

Dawson, Philip E. ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 36期

关键词：

aminoanilides; native chemical ligation; peptides; solid-phase synthesis; thioester;

D O I：

10.1002/anie.200705471

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Intramolecular activation of C-terminal peptides: Mildly activated N-acylurea peptides are readily formed on the solid support following chain assembly, avoiding over-activation of the C-terminus. The utility of these peptides is demonstrated by the native chemical ligation of unprotected peptides (see scheme; R = amino acid side chain). © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：6851 / 6855

页数：5

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