Enhancer-promoter specificity mediated by DPE or TATA core promoter motifs

被引:204
作者
Butler, JEF [1 ]
Kadonaga, JT [1 ]
机构
[1] Univ Calif San Diego, Mol Biol Sect, La Jolla, CA 92093 USA
关键词
enhancer; transcription; RNA polymerase II; DPE; TATA; core promoter;
D O I
10.1101/gad.924301
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
To investigate the basis for enhancer-promoter specificity, we compared the ability of enhancers to activate transcription in vivo from core promoters containing either downstream promoter element (DPE) or TATA box motifs. To eliminate position effects, we generated and analyzed pairs of sister Drosophila lines that contain a DPE- or TATA-dependent reporter gene at precisely the same genomic position relative to each enhancer. These studies revealed transcriptional enhancers that are specific for promoters that contain either DPE or TATA box elements. Thus, the core promoter not only mediates the initiation of transcription, but also functions as a regulatory element.
引用
收藏
页码:2515 / 2519
页数:5
相关论文
共 26 条
[1]  
[Anonymous], 2001, GENE TRANSCRIPTION M
[2]   P-ELEMENT-MEDIATED ENHANCER DETECTION - A VERSATILE METHOD TO STUDY DEVELOPMENT IN DROSOPHILA [J].
BELLEN, HJ ;
OKANE, CJ ;
WILSON, C ;
GROSSNIKLAUS, U ;
PEARSON, RK ;
GEHRING, WJ .
GENES & DEVELOPMENT, 1989, 3 (09) :1288-1300
[3]   AN AUTOREGULATORY ENHANCER ELEMENT OF THE DROSOPHILA HOMEOTIC GENE DEFORMED [J].
BERGSON, C ;
MCGINNIS, W .
EMBO JOURNAL, 1990, 9 (13) :4287-4297
[4]   SEARCHING FOR PATTERN AND MUTATION IN THE DROSOPHILA GENOME WITH A P-LACZ VECTOR [J].
BIER, E ;
VAESSIN, H ;
SHEPHERD, S ;
LEE, K ;
MCCALL, K ;
BARBEL, S ;
ACKERMAN, L ;
CARRETTO, R ;
UEMURA, T ;
GRELL, E ;
JAN, LY ;
JAN, YN .
GENES & DEVELOPMENT, 1989, 3 (09) :1273-1287
[5]   Going the distance: A current view of enhancer action [J].
Blackwood, EM ;
Kadonaga, JT .
SCIENCE, 1998, 281 (5373) :60-63
[6]   The downstream core promoter element, DPE, is conserved from Drosophila to humans and is recognized by TAF(II)60 of Drosophila [J].
Burke, TW ;
Kadonaga, JT .
GENES & DEVELOPMENT, 1997, 11 (22) :3020-3031
[7]   Drosophila TFIID binds to a conserved downstream basal promoter element that is present in many TATA-box-deficient promoters [J].
Burke, TW ;
Kadonaga, JT .
GENES & DEVELOPMENT, 1996, 10 (06) :711-724
[8]  
EMAMI KH, 1995, MOL CELL BIOL, V15, P5906
[9]   Transcription of the lymphocyte-specific terminal deoxynucleotidyltransferase gene requires a specific core promoter structure [J].
Garraway, IP ;
Semple, K ;
Smale, ST .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (09) :4336-4341
[10]   The downstream promoter element DPE appears to be as widely used as the TATA box in Drosophila core promoters [J].
Kutach, AK ;
Kadonaga, JT .
MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (13) :4754-4764