Spontaneous miniature outward currents in mechanically dissociated rat Meynert neurons

被引：20

作者：

Arima, J ^{[1
]}

Matsumoto, N ^{[1
]}

Kishimoto, K ^{[1
]}

Akaike, N ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Med Sci, Fukuoka 8128582, Japan

来源：

JOURNAL OF PHYSIOLOGY-LONDON | 2001年 / 534卷 / 01期

关键词：

D O I：

10.1111/j.1469-7793.2001.00099.x

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

1. Spontaneous miniature outward currents (SMOCs) were observed in mechanically dissociated rat Meynert neurons using nystatin perforated patch recordings under voltage-clamp conditions. 2. SMOCs were blocked by apamin, a selective blocker of small conductance Ca2+-activated Bf (XK) channels, but not by blockers for other types of Ca2+-activated K+ channel. 3. Ryanodine (10-100 muM) reduced both the amplitude and frequency of SMOCs. Caffeine (1 mM) increased the SMOC frequency. Blockers of the sarco/endoplasmic reticulum Ca2+-ATPase completely abolished SMOCs, indicating a requirement for functioning sarco/endoplasmic reticulum (SR/ER) Ca2+ stores. 4. Both Cd2+-containing and Ca2+-free solutions partially inhibited SMOC frequency, a result which suggests that Ca2+ influx contributes to, but is not essential for, SMOC generation. 5. Thus, SMOCs are XK currents linked to ryanodine- and caffeine-sensitive SR/ER Ca2+ stores, and are only indirectly influenced by extracellular Ca2+ influx. The development of this new, minimally invasive mechanical dissociation method has revealed that SMOCs are common in native CNS neurons.

引用

页码：99 / 107

页数：9

共 37 条

[31]

SAUNDERS HMH, 1992, J PHARMACOL EXP THER, V260, P1038

[32]

SEIDLER NW, 1989, J BIOL CHEM, V264, P17816

[33] Calcium release from internal stores is required for the generation of spontaneous hyperpolarizations in dopaminergic neurons of neonatal rats [J].