Robustness of two MALDI-TOF mass spectrometry systems for bacterial identification

被引：74

作者：

Carbonnelle, Etienne ^{[1
,2
]}

Grohs, Patrick

Jacquier, Herve ^{[3
,4
]}

Day, Nesrine

Tenza, Sylvie ^{[3
]}

Dewailly, Alexandra

Vissouarn, Odile ^{[3
]}

Rottman, Martin ^{[5
]}

Herrmann, Jean-Louis ^{[5
]}

Podglajen, Isabelle ^{[2
]}

Raskine, Laurent ^{[3
]}

机构：

[1] HEGP, AP HP, Microbiol Serv, F-75908 Paris, France

[2] Univ Paris 05, Fac Med, Paris, France

[3] Hop Lariboisiere, AP HP, Lariboisiere, France

[4] Univ Paris 07, Fac Med, UMR S 722, Paris, France

[5] Univ Versailles St Quentin En Yvelines, Versailles, France

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 89卷 / 02期

关键词：

Bacterial identification; MALDI-TOF-MS; Mass spectrometry; Clinical samples; DESORPTION IONIZATION-TIME; ASSISTED-LASER-DESORPTION/IONIZATION; ROUTINE IDENTIFICATION; BLOOD CULTURE; REVOLUTION; GENE;

D O I：

10.1016/j.mimet.2012.03.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MALDI-TOF-MS systems (Microflex-Bruker Daltonics/BioTyper (TM) and Axima-Assurance-Shimadzu/SARAMIS-AnagnosTec) were assessed for bacterial identification. Focusing on bacteria difficult to identify routinely, 296 strains were identified by molecular biology techniques as gold standard. MALDI-TOF-MS identification provided correct results at genus and species level for 94.9%, 83.4% and 83.8%, 65.9% with Biotyper and Saramis respectively. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：133 / 136

页数：4

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