Antioxidant sensors based on DNA-modified electrodes

被引：91

作者：

Liu, JF

Roussel, C

Lagger, G

Tacchini, P

Girault, HH

机构：

[1] Ecole Polytech Fed Lausanne, Lab Electrochim Phys & Analyt, CH-1015 Lausanne, Switzerland

[2] EDEL Therapeut SA, Ecole Polytech Fed Lausanne, CH-1015 Lausanne, Switzerland

来源：

ANALYTICAL CHEMISTRY | 2005年 / 77卷 / 23期

关键词：

D O I：

10.1021/ac0509298

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

TiO2/ITO modified electrodes were developed to quantitatively photooxidize adsorbed ds-DNA and to study the effect of antioxidants as ds-DNA protecting agents. TiO2 films are used for efficient ds-DNA immobilization, for dsDNA oxidation through photogenerated hydroxyl radicals, and as electrodes for amperometric sensing. The films, prepared by a sol-gel process, are deposited on ITO glass electrodes. Damages occurring after ds-DNA oxidation by ROS are detected by adding MB as an intercalant probe and by monitoring the electrochemical reduction current of the intercalated redox probe. The MB electrochemical signal is found to be sensitive enough to monitor ds-DNA structure changes, and the electrochemical sensor has been applied to the evaluation of the antioxidant properties of glutathione and gallic acid.

引用

收藏

页码：7687 / 7694

页数：8

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