Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)2(PVP)10]2+

被引：117

作者：

Dennany, L

Forster, RJ

White, B

Smyth, M

Rusling, JF ^{[1
]}

机构：

[1] Dublin City Univ, Sch Chem Sci, NCSR, Dublin 9, Ireland

[2] Univ Connecticut, Dept Chem, Storrs, CT 06269 USA

[3] Univ Connecticut, Ctr Hlth, Dept Pharmacol, Farmington, CT 06032 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 28期

关键词：

D O I：

10.1021/ja048615+

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.

引用

页码：8835 / 8841

页数：7

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