Enhancement of the sensitivity of surface plasmon resonance biosensor with colloidal gold labeling technique

被引：25

作者：

Gu, JH ^{[1
]}

Lü, H ^{[1
]}

Chen, YW ^{[1
]}

Liu, LY ^{[1
]}

Wang, P ^{[1
]}

Ma, JM ^{[1
]}

Lu, ZH ^{[1
]}

机构：

[1] Southeast Univ, Natl Lab Mol & Biomol Elect, Nanjing 210096, Peoples R China

来源：

SUPRAMOLECULAR SCIENCE | 1998年 / 5卷 / 5-6期

关键词：

immunosensor; surface plasmon resonance; colloidal gold;

D O I：

10.1016/S0968-5677(98)00105-9

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In our experiment, colloidal gold was used to enhance the signal obtained from the surface-plasmon-resonance biosensor. Fab' fragments of human Ige molecules were immobilized on the gold surface and reacted with the sheep-anti-human IgG(SAH-IgG) molecules in solution. It was found that when the colloidal gold labeling technique was introduced the sensitivity of surface plasmon resonance instruments increased by a factor of about 300. (C) 1998 Elsevier Science Limited. All rights reserved.

引用

页码：695 / 698

页数：4

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