Gene Expression Profile of the Regeneration Epithelium During Axolotl Limb Regeneration

被引：56

作者：

Campbell, Leah J. ^{[1
]}

Suarez-Castillo, Edna C. ^{[1
]}

Ortiz-Zuazaga, Humberto ^{[2
,3
]}

Knapp, Dunja ^{[4
]}

Tanaka, Elly M. ^{[4
]}

Crews, Craig M. ^{[1
,5
,6
]}

机构：

[1] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06511 USA

[2] Univ Puerto Rico, High Performance Comp Facil, San Juan, PR 00936 USA

[3] Univ Puerto Rico, Dept Comp Sci, San Juan, PR 00936 USA

[4] Ctr Regenerat Therapies, Dresden, Germany

[5] Yale Univ, Dept Chem, New Haven, CT 06511 USA

[6] Yale Univ, Dept Pharmacol, New Haven, CT 06511 USA

来源：

DEVELOPMENTAL DYNAMICS | 2011年 / 240卷 / 07期

关键词：

limb regeneration; urodele amphibian; gene expression microarray; regeneration epithelium; EGF-REPEAT; ADULT NEWT; MICROARRAY; NORMALIZATION; TRANSCRIPTION; RESOURCES; EPIDERMIS; PATTERNS;

D O I：

10.1002/dvdy.22669

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Urodele amphibians are unique among adult vertebrates in their ability to regenerate missing limbs. The process of limb regeneration requires several key tissues including a regeneration-competent wound epidermis called the regeneration epithelium (RE). We used microarray analysis to profile gene expression of the RE in the axolotl, a Mexican salamander. A list of 125 genes and expressed sequence tags (ESTs) showed a >= 1.5-fold expression in the RE than in a wound epidermis covering a lateral cuff wound. A subset of the RE ESTs and genes were further characterized for expression level changes over the time-course of regeneration. This study provides the first large scale identification of specific gene expression in the RE. Developmental Dynamics 240:1826-1840, 2011. (C) 2011 Wiley-Liss, Inc.

引用

页码：1826 / 1840

页数：15

共 55 条

[1] Desmoglein 4 is expressed in highly differentiated keratinocytes and trichocytes in human epidermis and hair follicle [J].

Bazzi, H ;

Getz, A ;

Mahoney, MG ;

Ishida-Yamamoto, A ;

Langbein, L ;

Wahl, JK ;

Christiano, AM .

DIFFERENTIATION, 2006, 74 (2-3) :129-140

[2] CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].

BENJAMINI, Y ;

HOCHBERG, Y .

JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300

[3] The thyroid hormone-induced tail resorption program during Xenopus laevis metamorphosis [J].

Brown, DD ;

Wang, Z ;

Furlow, JD ;

Kanamori, A ;

Schwartzman, RA ;

Remo, BF ;

Pinder, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (05) :1924-1929

[4] MAEG, an EGF-repeat containing gene, is a new marker associated with dermatome specification and morphogenesis of its derivatives [J].

Buchner, G ;

Broccoli, V ;

Bulfone, A ;

Orfanelli, U ;

Gattuso, C ;

Ballabio, A ;

Franco, B .

MECHANISMS OF DEVELOPMENT, 2000, 98 (1-2) :179-182

[5] Identification of a new EGF-repeat-containing gene from human Xp22: A candidate for developmental disorders [J].

Buchner, G ;

Orfanelli, U ;

Quaderi, N ;

Bassi, MT ;

Andolfi, G ;

Ballabio, A ;

Franco, B .

GENOMICS, 2000, 65 (01) :16-23

[6] Wound epidermis formation and function in urodele amphibian limb regeneration [J].

Campbell, L. J. ;

Crews, C. M. .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2008, 65 (01) :73-79

[7]

Carlson MRJ, 1998, J EXP ZOOL, V282, P715, DOI 10.1002/(SICI)1097-010X(19981215)282:6<715::AID-JEZ7>3.0.CO

[8]

2-F

[9] Expression of fibroblast growth factors 4, 8, and 10 in limbs, flanks, and blastemas of Ambystoma [J].

Christensen, RN ;

Weinstein, M ;

Tassava, RA .

DEVELOPMENTAL DYNAMICS, 2002, 223 (02) :193-203

[10] The newt ortholog of CD59 is implicated in proximodistal identity during amphibian limb regeneration [J].

da Silva, SM ;

Gates, PB ;

Brockes, JP .

DEVELOPMENTAL CELL, 2002, 3 (04) :547-555

← 1 2 3 4 5 6 →