Domain annotation of trimeric autotransporter adhesins - daTAA

被引：82

作者：

Szczesny, Pawel ^{[1
,2
]}

Lupas, Andrei ^{[1
]}

机构：

[1] Max Planck Inst Dev Biol, Dept Protein Evolut, D-72076 Tubingen, Germany

[2] Inst Biochem & Biophys PAS, Dept Bioinformat, PL-02106 Warsaw, Poland

来源：

BIOINFORMATICS | 2008年 / 24卷 / 10期

关键词：

D O I：

10.1093/bioinformatics/btn118

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Motivation: Trimeric autotransporter adhesins (TAAs), such as Yersinia YadA, Neisseria NadA, Moraxella UspAs, Haemophilus Hia and Bartonella BadA, are important pathogenicity factors of proteobacteria. Their high sequence diversity and distinct mosaic-like structure lead to difficulties in the annotation of their sequences. These stem from the large number of short repeats, the presence of compositionally unusual coiled-coils, fuzzy domain boundaries and regions of seemingly low sequence complexity. Results: We have developed a workflow, named daTAA, for the accurate domain annotation of TAAs. Its core consists of manually curated alignments and of knowledge-based rules that enhance assignments made by sequence similarity. Compared to general domain annotation servers such as PFAM, daTAA captures more domains and provides more sensitive domain detection, as well as integrated and detailed coiled-coil assignments.

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收藏

页码：1251 / 1256

页数：6

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