Monovalent, reduced-size quantum dots for imaging receptors on living cells

被引：244

作者：

Howarth, Mark ^{[1
]}

Liu, Wenhao ^{[1
]}

Puthenveetil, Sujiet ^{[1
]}

Zheng, Yi ^{[1
]}

Marshall, Lisa F. ^{[1
]}

Schmidt, Michael M. ^{[2
,3
]}

Wittrup, K. Dane ^{[2
,3
]}

Bawendi, Moungi G. ^{[1
]}

Ting, Alice Y. ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

[2] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[3] MIT, Div Biol Engn, Cambridge, MA 02139 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 05期

关键词：

D O I：

10.1038/NMETH.1206

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We describe a method to generate monovalent quantum dots (QDs) using agarose gel electrophoresis. We passivated QDs with a carboxy-terminated polyethylene-glycol ligand, yielding particles with half the diameter of commercial QDs, which we conjugated to a single copy of a high-affinity targeting moiety (monovalent streptavidin or antibody to carcinoembryonic antigen) to label cell-surface proteins. The small size improved access of QD-labeled glutamate receptors to neuronal synapses, and monovalency prevented EphA3 tyrosine kinase activation.

引用

收藏

页码：397 / 399

页数：3

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