Genomic analysis of biochemical function

被引：12

作者：

Grayhack, EJ ^{[1
]}

Phizicky, EM ^{[1
]}

机构：

[1] Univ Rochester, Sch Med & Dent, Dept Biochem & Biophys, Rochester, NY 14642 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2001年 / 5卷 / 01期

关键词：

D O I：

10.1016/S1367-5931(00)00169-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Establishing the linkage between an individual biochemical activity and the gene(s) specifying that activity has been facilitated by advances in mass spectrometry and affinity purification methods. In addition, a genomic protein array has been produced in yeast by fusing each yeast open reading frame to glutathione-S-transferase thus linking each protein With its cognate gene. Purification and biochemical assay of pools of glutathione-S-transferase-open-reading-frame proteins allows analysis of the entire proteome for biochemical activities, followed by simple deconvolution to identify the responsible open reading frame. An alternative method to analyze large sets of proteins is the use of protein microarrays in which over 10,000 individual proteins can be immobilized and: assayed on a single slide.

引用

页码：34 / 39

页数：6

共 19 条

[1] Specific proteolysis of the kinase protein kinase C-related kinase 2 by caspase-3 during apoptosis - Identification by a novel, small pool expression cloning strategy [J].

Cryns, VL ;

Byun, Y ;

Rana, A ;

Mellor, H ;

Lustig, KD ;

Ghanem, L ;

Parker, PJ ;

Kirschner, MW ;

Yuan, JY .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (47) :29449-29453

[2] Mechanistically diverse enzyme superfamilies: the importance of chemistry in the evolution of catalysis [J].

Gerlt, JA ;

Babbitt, PC .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1998, 2 (05) :607-612

[3] Quantitative analysis of complex protein mixtures using isotope-coded affinity tags [J].

Gygi, SP ;

Rist, B ;

Gerber, SA ;

Turecek, F ;

Gelb, MH ;

Aebersold, R .

NATURE BIOTECHNOLOGY, 1999, 17 (10) :994-999

[4] Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries [J].

Hanes, J ;

Jermutus, L ;

Weber-Bornhauser, S ;

Bosshard, HR ;

Plückthun, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (24) :14130-14135

[5] Identification of a new uracil-DNA glycosylase family by expression cloning using synthetic inhibitors [J].

Haushalter, KA ;

Stukenberg, PT ;

Kirschner, MW ;

Verdine, GL .

CURRENT BIOLOGY, 1999, 9 (04) :174-185

[6] Moonlighting proteins [J].

Jeffery, CJ .

TRENDS IN BIOCHEMICAL SCIENCES, 1999, 24 (01) :8-11

[7] Direct analysis of protein complexes using mass spectrometry [J].

Link, AJ ;

Eng, J ;

Schieltz, DM ;

Carmack, E ;

Mize, GJ ;

Morris, DR ;

Garvik, BM ;

Yates, JR .

NATURE BIOTECHNOLOGY, 1999, 17 (07) :676-682

[8]

Lustig KD, 1997, METHOD ENZYMOL, V283, P83

[9] A combined algorithm for genome-wide prediction of protein function [J].

Marcotte, EM ;

Pellegrini, M ;

Thompson, MJ ;

Yeates, TO ;

Eisenberg, D .

NATURE, 1999, 402 (6757) :83-86

[10] Cloning of Mix-related homeodomain proteins using fast retrieval of gel shift activities, (FROGS), a technique for the isolation of DNA-binding proteins [J].

Mead, PE ;

Zhou, Y ;

Lustig, KD ;

Huber, TL ;

Kirschner, MW ;

Zon, LI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (19) :11251-11256

← 1 2 →