Protein cross-linking mediated by metalloporphyrins

被引：14

作者：

Campbell, LA

Kodadek, T

Brown, KC

机构：

[1] Univ Texas, SW Med Ctr, Ctr Biomed Invent, Dept Internal Med, Dallas, TX 75235 USA

[2] Univ Texas, SW Med Ctr, Ctr Biomed Invent, Dept Biochem, Dallas, TX 75235 USA

[3] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 1998年 / 6卷 / 08期

关键词：

protein-protein interactions; cross-linking; oxidation; porphyrin; chemical biology;

D O I：

10.1016/S0968-0896(98)00120-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A biomimetic protein cross-linking reaction is described which employs oxidatively-activated manganese and iron porphyrins as the reactive species. A wide range of proteins cross-link under these conditions, but only if they are intimately associated in solution. The reaction is rapid, efficient, and will be useful for the suprastructural analysis of multiprotein complexes. (C) 1998 Published by Elsevier Science Ltd. All rights reserved.

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收藏

页码：1301 / 1307

页数：7

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