Fusarium oxysporum gas1 encodes a putative β-1,3-glucanosyltransferase required for virulence on tomato plants

被引:57
作者
Caracuel, Z [1 ]
Martínez-Rocha, AL [1 ]
Di Pietro, A [1 ]
Madrid, MP [1 ]
Roncero, MIG [1 ]
机构
[1] Univ Cordoba, Dept Genet, E-14071 Cordoba, Spain
关键词
glucan;
D O I
10.1094/MPMI-18-1140
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Glycosylphosphatidylinositol-anchored (beta)-1,3-glucanosyltransferases play active roles in fungal cell wall biosynthesis and morphogenesis and have been implicated in virulence on mammals. The role of beta-1,3-glucanosyltransferases in pathogenesis to plants has not been explored so far. Here, we report the cloning and mutational analysis of the gas1 gene encoding a putative beta-1,3-glucanosyltransferase from the vascular wilt fungus Fusarium oxysporum. In contrast to Candida albicans, expression of gas1 in E oxysporum was independent of ambient pH and of the pH response transcription factor PacC. Gene knockout mutants lacking a functional gas1 allele grew in a way similar to the wildtype strain in submerged culture but exhibited restricted colony growth on solid substrates. The restricted growth phenotype was relieved by the osmotic stabilizer sorbitol, indicating that it may be related to structural alterations in the cell wall. Consistent with this hypothesis, Delta gas1 mutants exhibited enhanced resistance to cell wall-degrading enzymes and increased transcript levels of chsV and rho1, encoding a class V chitin synthase and a small monomeric G protein, respectively. The Delta gas1 mutants showed dramatically reduced virulence on tomato, both in a root infection assay and in a fruit tissue-invasion model, thus providing the first evidence for an essential role of fungal 01,3-glucanosyltransferases during plant infection.
引用
收藏
页码:1140 / 1147
页数:8
相关论文
共 42 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]   Cell wall integrity modulates RHO1 activity via the exchange factor ROM2 [J].
Bickle, M ;
Delley, PA ;
Schmidt, A ;
Hall, MN .
EMBO JOURNAL, 1998, 17 (08) :2235-2245
[3]   Distinct and redundant roles of the two protein kinase A isoforms Tpk1p and Tpk2p in morphogenesis and growth of Candida albicans [J].
Bockmühl, DP ;
Krishnamurthy, S ;
Gerads, M ;
Sonneborn, A ;
Ernst, JF .
MOLECULAR MICROBIOLOGY, 2001, 42 (05) :1243-1257
[4]   The pH signalling transcription factor PacC controls virulence in the plant pathogen Fusarium oxysporum [J].
Caracuel, Z ;
Roncero, MIG ;
Espeso, EA ;
González-Verdejo, CI ;
García-Maceira, FI ;
Di Pietro, A .
MOLECULAR MICROBIOLOGY, 2003, 48 (03) :765-779
[5]   Maintenance of cell integrity in the gas1 mutant of Saccharomyces cerevisiae requires the Chs3p-targeting and activation pathway and involves an unusual Chs3p localization [J].
Carotti, C ;
Ferrario, L ;
Roncero, C ;
Valdivieso, MH ;
Duran, A ;
Popolo, L .
YEAST, 2002, 19 (13) :1113-1124
[6]   Properties of various RHO1 mutant alleles of Cryptococcus neoformans [J].
Chang, YC ;
Penoyer, LA .
JOURNAL OF BACTERIOLOGY, 2000, 182 (17) :4987-4991
[7]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[8]   RIM101-dependent and -independent pathways govern pH responses in Candida albicans [J].
Davis, D ;
Wilson, RB ;
Mitchell, AP .
MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (03) :971-978
[9]   The pH of the host niche controls gene expression in and virulence of Candida albicans [J].
De Bernardis, F ;
Mühlschlegel, FA ;
Cassone, A ;
Fonzi, WA .
INFECTION AND IMMUNITY, 1998, 66 (07) :3317-3325
[10]  
Di Pietro A, 2003, MOL PLANT PATHOL, V4, P315, DOI [10.1046/j.1364-3703.2003.00180.x, 10.1046/J.1364-3703.2003.00180.X]