Gene transfer into retinal ganglion cells by in vivo electroporation: a new approach

被引：70

作者：

Dezawa, M

Takano, M

Negishi, H

Mo, XF

Oshitari, T

Sawada, H

机构：

[1] Yokohama City Univ, Sch Med, Dept Anat, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[2] Yokohama City Univ, Sch Med, Dept Ophthalmol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[3] Chiba Univ, Sch Med, Dept Ophthalmol, Chuo Ku, Chiba 2608670, Japan

来源：

MICRON | 2002年 / 33卷 / 01期

关键词：

eye; retina; vision; electric pulse; green fluorescent protein; in vivo gene therapy;

D O I：

10.1016/S0968-4328(01)00002-6

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We developed a new in vivo electroporation. method to deliver genes into retinal ganglion cells (RGCs). Efficiency and degree of tissue damage were evaluated using green fluorescent protein (GFP) gene and TUNEL. Soon after the intravitreous injection of the GFP gene, electroporation (five electric pulses of 99 ms duration each and 12 V/cm. delivered twice 5 min apart) was carried out on the adult rat eyeball with the aid of tweezer-type disc electrodes attached to corneal (cathode) and scleral (anode) surfaces. GFP expression, exhibiting a maximum on day 7, was detectable for up to 21 days. DiI retrograde labeling of RGCs showed that 41.5% of the total ganglion cells in the electroinjected area were GFP-positive. Therefore, this new method may be a useful tool for the delivery of genes into RGCs. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：1 / 6

页数：6

共 24 条

[1] Assessing the potential of skin electroporation for the delivery of protein- and gene-based drugs [J].