Protein phosphatase 2A is required for the initiation of chromosomal DNA replication

被引：75

作者：

Lin, XH

Walter, J

Scheidtmann, K

Ohst, K

Newport, J

Walter, G ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Pathol, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA

[3] Univ Bonn, Inst Genet, D-53117 Bonn, Germany

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 25期

关键词：

D O I：

10.1073/pnas.95.25.14693

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Protein phosphatase 2A (PP2A) is an abundant, multifunctional serine/threonine-specific phosphatase that stimulates simian virus 40 DNA replication. The question as to whether chromosomal DNA replication also depends on PP2A was addressed by using a cell-free replication system derived from Xenopus laevis eggs. Immunodepletion of PP2A from Xenopus egg extract resulted in strong inhibition of DNA replication. PP2A was required for the initiation of replication but not for the elongation of previously engaged replication forks. Therefore, the initiation of chromosomal DNA replication depends not only on phosphorylation by protein kinases but also on dephosphorylation by PP2A.

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页码：14693 / 14698

页数：6

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