Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi

被引：91

作者：

Kumaran, D

Eswaramoorthy, S

Luft, BJ

Koide, S

Dunn, JJ

Lawson, CL

Swaminathan, S ^{[1
]}

机构：

[1] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA

[2] SUNY Stony Brook, Sch Med, Div Infect Dis, Stony Brook, NY USA

[3] Univ Rochester, Med Ctr, Dept Biochem & Biophys, Rochester, NY 14642 USA

[4] Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA

来源：

EMBO JOURNAL | 2001年 / 20卷 / 05期

关键词：

Lyme disease; OspC; 3D structure; X-ray diffraction;

D O I：

10.1093/emboj/20.5.971

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Outer surface protein C (OspC) is a major antigen on the surface of the Lyme disease spirochete, Borrelia burgdorferi, when it is being transmitted to humans. Crystal structures of OspC have been determined for strains HB19 and B31 to 1.8 and 2.5 Angstrom resolution, respectively. The three-dimensional structure is predominantly helical. This is in contrast to the structure of OspA, a major surface protein mainly present when spirochetes are residing in the midgut of unfed ticks, which is mostly P-sheet. The surface of OspC that would project away from the spirochete's membrane has a region of strong negative electrostatic potential which may be involved in binding to positively charged host ligands. This feature is present only on OspCs from strains known to cause invasive human disease.

引用

页码：971 / 978

页数：8

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