Prussian blue modified amperometric FIA biosensor:: one-step immunoassay for α-fetoprotein

被引：33

作者：

Guan, JG ^{[1
]}

Miao, YQ

Chen, JR

机构：

[1] Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Wuhan 430070, Peoples R China

[2] Zhejiang Normal Univ, Sch Life & Environm Sci, Jinhua 321004, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2004年 / 19卷 / 08期

关键词：

alpha-fetoprotein; biosensor; Prussian blue;

D O I：

10.1016/j.bios.2003.08.006

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The aim of this study was to develop a rapid method to measure alpha-fetoprotein (AFP) in human serum by use of one-step sandwich enzyme-linked immunosorbent assay (ELISA)-based immunobiosensor with disposable screen-printed carbon electrode technology. Prussian blue (PB) was deposited using cyclic voltammetry (CV) on the surface of electrode to catalyze H2O2 from the reaction of glucose oxidase. It took only about 30 min to complete the whole experimental procedure through flow injection analysis (FIA). A detection range obtained is in the ran-e from 5 to 500 ng/ml AFP. This detection seems to be quick, reliable and practical. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：789 / 794

页数：6

共 18 条

[1] Chou SF, 2002, CLIN CHEM, V48, P913
[2] Christiansen M, 2001, SCAND J CLIN LAB INV, V61, P205
[3] Highly sensitive amperometric enzyme immunoassay for alpha-fetoprotein in human serum
DellaCiana, L
Bernacca, G
DeNitti, C
Massaglia, A
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1996, 193 (01) : 51 - 62
[4] JAVADPOUR N, 1980, CANCER-AM CANCER SOC, V45, P2166, DOI 10.1002/1097-0142(19800415)45:8<2166::AID-CNCR2820450826>3.0.CO
[5] 2-5
[6] The electrocatalytic activity of Prussian blue in hydrogen peroxide reduction studied using a wall-jet electrode with continuous flow
Karyakin, AA
Karyakina, EE
Gorton, L
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1998, 456 (1-2) : 97 - 104
[7] Karyakin AA, 2001, ELECTROANAL, V13, P813, DOI 10.1002/1521-4109(200106)13:10<813::AID-ELAN813>3.0.CO
[8] 2-Z
[9] Prussian-Blue-based amperometric biosensors in flow-injection analysis
Karyakin, AA
Karyakina, EE
Gorton, L
[J]. TALANTA, 1996, 43 (09) : 1597 - 1606
[10] PRUSSIAN BLUE BASED FIRST-GENERATION BIOSENSOR - A SENSITIVE AMPEROMETRIC ELECTRODE FOR GLUCOSE
KARYAKIN, AA
GITELMACHER, OV
KARYAKINA, EE
[J]. ANALYTICAL CHEMISTRY, 1995, 67 (14) : 2419 - 2423

← 1 2 →