Integration of various stacking processes in carrier ampholyte-based capillary electrophoresis

被引：17

作者：

Busnel, Jean-Marc ^{[1
,2
]}

Le Saux, Thomas ^{[3
,4
]}

Descroix, Stephanie ^{[2
]}

Girault, Hubert H. ^{[1
]}

Hennion, Marie-Claire ^{[2
]}

Terabe, Shigeru ^{[3
]}

Peltre, Gabriel ^{[2
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Electrochim Phys & Analyt, EPFL SB ISIC LEPA, CH-1015 Lausanne, Switzerland

[2] ESPCI, CNRS, UMR 7121, Lab Environm Chim Analyt, F-75005 Paris, France

[3] Univ Hyogo, Grad Sch Mat Sci, Kamigori, Hyogo 6781297, Japan

[4] Ecole Normale Super, CNRS, UPMC, Dept Chim, F-75231 Paris, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1182卷 / 02期

基金：

日本学术振兴会;

关键词：

carrier ampholytes; CABCE; FESS; FESI; EKS;

D O I：

10.1016/j.chroma.2007.12.079

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Field-enhanced sample stacking, field-enhanced sample injection as well as electrokinetic supercharging have been successfully integrated in carrier ampholyte-based capillary electrophoresis. Through the analysis of different test sample mixtures, it has been shown that the carrier ampholyte-based background electrolytes, in spite of their very low conductivity, allow efficient online preconcentration of analytes by field-amplified techniques. Sensitivity enhancement factors of the same magnitude as those usually encountered with classical conductive background electrolytes have been obtained in such carrier ampholyte-based buffers. Depending on the online preconcentration method that has been integrated, sensitivity enhancement factors between 50 and several thousands have been reached. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：226 / 232

页数：7

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