Identification of extended-spectrum -lactamase and quinolone resistance genes in Escherichia coli isolated from hospital wastewater from central India

被引：60

作者：

Diwan, Vishal ^{[1
,2
]}

Chandran, Salesh P. ^{[3
,4
]}

Tamhankar, A. J. ^{[5
]}

Lundborg, Cecilia Stalsby ^{[2
]}

Macaden, Ragini ^{[3
]}

机构：

[1] RD Gardi Med Coll, Dept Publ Hlth & Environm, Ujjain, Madhya Pradesh, India

[2] Karolinska Inst, Dept Publ Hlth Sci, Div Global Hlth IHCAR, SE-17177 Stockholm, Sweden

[3] St Johns Res Inst, Infect Dis Unit, Bangalore, Karnataka, India

[4] St Johns Med Coll, Dept Microbiol, Bangalore, Karnataka, India

[5] RD Gardi Med Coll, Indian Initiat Management Antibiot Resistance IIM, Dept Environm Med, Ujjain, Madhya Pradesh, India

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2012年 / 67卷 / 04期

基金：

瑞典研究理事会;

关键词：

bla(CTX-M); bla(TEM); bla(SHV); qnr genes; ANTIBIOTICS;

D O I：

10.1093/jac/dkr564

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

To investigate the presence of antibiotic resistance genes (ARGs), related to commonly used -lactams and quinolones, in Escherichia coli present in hospital wastewater in central India. Cefotaxime- and ciprofloxacin-resistant E. coli isolates from hospital-associated wastewater samples were collected from two tertiary care hospitals in the Ujjain district of India during 200809. The presence of bla(CTX-M), bla(TEM,) bla(SHV), qnrA, qnrB, qnrS, aac(6)-Ib-cr and qepA genes was detected by PCR and sequencing. Twenty-five E. coli isolates were extended-spectrum -lactamase (ESBL) positive. bla(CTX-M-15) and bla(TEM-1) genes were identified in 21 and 16 ESBL-positive isolates by PCR, respectively. Amongst 30 fluoroquinolone-resistant E. coli isolates, aac(6)-Ib-cr was detected in 27 isolates, qnrA in 1 isolate, qnrB in 2 isolates and qepA in 3 isolates. bla(CTX-M-15,) bla(TEM-1), aac(6)-Ib-cr, qnrA, qnrB and qepA genes are present in E. coli occurring in hospital wastewater in central India.

引用

页码：857 / 859

页数：3

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