Mg Protoporphyrin Monomethylester Cyclase Deficiency and Effects on Tetrapyrrole Metabolism in Different Light Conditions

被引:45
作者
Peter, Enrico [1 ]
Rothbart, Maxi [1 ]
Oelze, Marie-Luise [2 ]
Shalygo, Nikolai [1 ]
Dietz, Karl-Josef [2 ]
Grimm, Bernhard [1 ]
机构
[1] Humboldt Univ, Inst Biol Plant Physiol, D-10115 Berlin, Germany
[2] Univ Bielefeld, D-33615 Bielefeld, Germany
关键词
Chlorophyll; Mg protoporphyrin monomethylester cyclase; Photooxidation; Photosensitization; Tetrapyrrole biosynthesis; Tobacco; SPINACH SPINACIA-OLERACEA; ESTER OXIDATIVE CYCLASE; RNA-SYNTHESIS LEADS; ARABIDOPSIS-THALIANA; HYDROGEN-PEROXIDE; STRESS RESPONSES; GENE-EXPRESSION; SINGLET OXYGEN; CHLAMYDOMONAS-REINHARDTII; CHLOROPHYLL BIOSYNTHESIS;
D O I
10.1093/pcp/pcq071
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Mg protoporphyrin monomethylester (MgProtoME) cyclase catalyzes isocyclic ring formation to form divinyl protochlorophyllide. The CHL27 protein is part of the cyclase complex. Deficiency of CHL27 has been previously reported to compromise photosynthesis and nuclear gene expression. In a comprehensive analysis of different CHL27 antisense tobacco lines grown under different light conditions, the physiological consequences of gradually reduced CHL27 expression on the tetrapyrrole biosynthetic pathway were explored. Excessive amounts of MgProtoME, the substrate of the cyclase reaction, accumulated in response to the reduced CHL27 content. Moreover, 5-aminolevulinic acid (ALA) synthesis, Mg chelatase and Mg protoporphyrin methyltransferase activities were reduced in transgenic plants. Compared with growth under continuous light exposure, the CHL27-deficient plants showed a stronger reduction in Chl content, cell death and leaf necrosis during diurnal light/dark cycles. This photooxidative phenotype correlated with a rapidly increasing MgProtoME steady-state level at the beginning of each light period. In contrast, the same transformants grown under continuous light exposure possessed a permanently elevated amount of MgProtoME. Its lower phototoxicity correlated with increased activities of ascorbate peroxidase and catalase, and a higher amount of reduced ascorbate. It is proposed that improved stress acclimation during continuous light in comparison with light-dark growth increases the capacity to prevent photo-oxidation by excess tetrapyrrole precursors and lowers the susceptibility to secondary photodynamic damage.
引用
收藏
页码:1229 / 1241
页数:13
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