Quantification of substratum contact required for initiation of Colletotrichum graminicola appressoria

被引:22
作者
Apoga, D
Barnard, J
Craighead, HG
Hoch, HC [1 ]
机构
[1] Cornell Univ, New York State Agr Expt Stn, Dept Plant Pathol, Geneva, NY 14456 USA
[2] Cornell Univ, New York State Agr Expt Stn, Comp Ctr, Geneva, NY 14456 USA
[3] Cornell Univ, Sch Appl & Engn Phys, Ithaca, NY 14850 USA
基金
美国国家科学基金会;
关键词
appressoria; adhesion; bovine serum albumin; interference-reflection microscopy; microfabrication; pillared surface; surface contact; Colletotrichum graminicola;
D O I
10.1016/j.fgb.2003.10.001
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Colletotrichum graminicola, like many plant pathogenic fungi develop appressoria on germling apices, to facilitate penetration of their host. Induction of these structures occurs after contact with the host surface has been established by the germling. Surface contact and subsequent development of appressoria by germlings of C graminicola was assessed using interference-reflection microscopy (IRM) and microfabricated pillared silicon substrata. Observations with IRM revealed that under low nutrient conditions, 90% of the germlings developed appressoria once they established 4.5 mum of continuous contact with the substratum. Substrata bearing pillars less than or equal to 5 mum in width supported less than or equal to 10% appressoria; however, as pillar width was increased the percentage of appressoria formed increased in a sigmoid fashion to a maximum of 80%. The percentage of appressoria produced experimentally on these surfaces was compared to data sets generated from a model designed to calculate the probability of appressorium development on similar pillar arrays at various germ tube contact lengths. These results indicate that germ tubes of C graminicola require more than 4 mum of continuous contact with a hydrophobic substratum for induction of appressoria. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:1 / 12
页数:12
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