Lipopolysaccharide structures of Helicobacter pylori wild-type strain 26695 and 26695 HP0826::Kan mutant devoid of the O-chain polysaccharide component

被引：23

作者：

Altman, Eleonora ^{[1
]}

Chandan, Vandana ^{[1
]}

Li, Jianjun ^{[1
]}

Vinogradov, Evgeny ^{[1
]}

机构：

[1] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

来源：

CARBOHYDRATE RESEARCH | 2011年 / 346卷 / 15期

关键词：

Helicobacter pylori; LPS; Lipopolysaccharide; Structure; NMR; GLYCOTYPE FAMILIES; MOLECULAR MIMICRY; INFECTION; MONOSACCHARIDES; IDENTIFICATION; COLONIZATION; ANTIGENS; EPITOPE; CANCER;

D O I：

10.1016/j.carres.2011.06.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

We describe a re-investigation of the structure of the lipopolysaccharide (LPS) from Helicobacter pylori genomic strain 26695 and its corresponding HP0826::Kan mutant lacking the O-chain component based on the in-depth NMR analysis of the oligosaccharide products obtained through the use of various degradation procedures performed on the purified LPS from both strains, as well as CE-MS data. New structural evidence indicates the presence of the linear arrangement of glucan and heptan portions of the LPS attached through -6-alpha-DDHep-3-alpha-L-Fuc-3-beta-GlcNAc- fragment to the inner core DD-heptose residue. This structure differs from previously reported structures of the H. pylori 26695 LPS in several aspects. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.

引用

页码：2437 / 2444

页数：8

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