Resonance assignment of 13C-15N-labeled snake neurotoxin II from Naja oxiana

被引：11

作者：

Bocharov, EV ^{[1
]}

Lyukmanova, EN ^{[1
]}

Ermolyuk, YS ^{[1
]}

Schulga, AA ^{[1
]}

Pluzhnikov, KA ^{[1
]}

Dolgikh, DA ^{[1
]}

Kirpichnikov, MP ^{[1
]}

Arseniev, AS ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

来源：

APPLIED MAGNETIC RESONANCE | 2003年 / 24卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

D O I：

10.1007/BF03166664

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 [原子与分子物理]; 070304 [物理化学]; 081704 [应用化学]; 1406 [纳米科学与工程];

摘要：

Neurotoxin II from Naja oxiana venom is a short-chain snake curaremimetic neurotoxin containing four disulfide bonds. We obtained C-13-N-15-labeled neurotoxin II to study its internal dynamics and surface properties with atomic resolution. The recombinant protein has the native spatial structure and is biologically active. The nearly complete assignment of H-1, C-13 and N-15 resonances for neurotoxin II was obtained by heteronuclear triple-resonance nuclear magnetic resonance spectroscopy. Analysis of the secondary chemical shifts of the H-1(alpha), C-13(alpha), C-13(beta) and (CO)-C-13 nuclei reveal their strong correlation with the protein secondary structure.

引用

页码：247 / 254

页数：8

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