New coumarins from the roots of Angelica dahurica var. formosana cv. Chuanbaizhi and their inhibition on NO production in LPS-activated RAW264.7 cells

被引：30

作者：

Deng, Gai-Gai ^{[1
]}

Wei, Wei ^{[1
]}

Yang, Xiu-Wei ^{[1
]}

Zhang, You-Bo ^{[1
]}

Xu, Wei ^{[1
]}

Gong, Ning-Bo ^{[2
]}

Lu, Yang ^{[2
]}

Wang, Feng-Feng ^{[2
]}

机构：

[1] Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs,Dept Nat Med, Beijing 100191, Peoples R China

[2] Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China

来源：

FITOTERAPIA | 2015年 / 101卷

基金：

中国国家自然科学基金;

关键词：

Angelica dahurica var. formosana cv; Chuanbaizhi; Coumarins; (-)-Hydroxydecursinol; (+/-)-Dahuribiscoumarin; Nitric oxide; Inducible nitric oxide synthase; NITRIC-OXIDE SYNTHASE; IMPERATORIN; EXPRESSION; ISOIMPERATORIN; PHELLOPTERIN; INJURY; SEEDS;

D O I：

10.1016/j.fitote.2015.01.016

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

A new linear pyranocoumarin named (-)-hydroxydecursinol (1) and a new biscoumarin named (+/-)-dahuribiscoumarin (2), together with six known compounds isoimperatorin (3), imperatorin (4), phellopterin (5), isodemethylfuropinarine (6), demethylfuropinarine (7), and (+)-decursinol (8) were isolated from the 75% ethanolic extract of the roots of Angelica dahurica var. formosana cv. Chuanbaizhi. Their structures were elucidated by extensive spectroscopic techniques, including 2D NMR spectroscopy and mass spectrometry, and the structure of 2 was confirmed by single-crystal X-ray diffraction. All of the isolated compounds were evaluated for the inhibition against nitric oxide (NO) production in the lipopolysaccharide (LPS)-activated RAW264.7 macrophage cell line, and exhibited the inhibitory activity on NO production in a concentration-dependent manner. Furthermore, real-time PCR analysis revealed that compounds 2, 5-8 could significantly suppress the expression levels of inducible nitric oxide synthase mRNA in a concentration-dependent manner. And their primary structure-activity relationships of NO inhibitory effects were also briefly discussed. These compounds are potential candidates for further bioassay studies to determine their suitability as drug leads. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：194 / 200

页数：7

共 36 条

[1]

Identification and biological evaluation of grapefruit oil components as potential novel efflux pump modulators in methicillin-resistant Staphylococcus aureus bacterial strains [J].