The transcriptional coactivator SAYP is a trithorax group signature subunit of the PBAP chromatin remodeling complex

被引:66
作者
Chalkley, Gillian E. [1 ]
Moshkin, Yuri M. [1 ]
Langenberg, Karin [1 ]
Bezstarosti, Karel [2 ]
Blastyak, Andras [3 ]
Gyurkovics, Henrik [3 ]
Demmers, Jeroen A. A. [2 ]
Verrijzer, C. Peter [1 ]
机构
[1] Erasmus Univ, Med Ctr, Ctr Biomed Genet, Dept Biochem, NL-3000 DR Rotterdam, Netherlands
[2] Erasmus Univ, Med Ctr, Proteom Ctr, NL-3000 DR Rotterdam, Netherlands
[3] Hungarian Acad Sci, Biol Res Ctr, Genet Inst, H-6701 Szeged, Hungary
关键词
D O I
10.1128/MCB.02217-07
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
SWI/SNF ATP-dependent chromatin remodeling complexes (remodelers) perform critical functions in eukaryotic gene expression control. BAP and PBAP are the fly representatives of the two evolutionarily conserved major subclasses of SWI/SNF remodelers. Both complexes share seven core subunits, including the Brahma ATPase, but differ in a few signature subunits; POLYBROMO and BAP170 specify PBAP, whereas OSA defines BAP. Here, we show that the transcriptional coactivator and PHD finger protein SAYP is a novel PBAP subunit. Biochemical analysis established that SAYP is tightly associated with PBAP but absent from BAP. SAYP, POLYBROMO, and BAP170 display an intimately overlapping distribution on larval salivary gland polytene chromosomes. Genome-wide expression analysis revealed that SAYP is critical for PBAP-dependent transcription. SAYP is required for normal development and interacts genetically with core- and PBAP-selective subunits. Genetic analysis suggested that, like BAP, PBAP also counteracts Polycomb silencing. SAYP appears to be a key architectural component required for the integrity and association of the PBAP-specific module. We conclude that SAYP is a signature subunit that plays a major role in the functional specificity of the PBAP holoenzyme.
引用
收藏
页码:2920 / 2929
页数:10
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