Archaeal communities in High Arctic wetlands at Spitsbergen, Norway (78°N) as characterized by 16S rRNA gene fingerprinting

被引:74
作者
Hoj, L
Olsen, RA
Torsvik, VL
机构
[1] Univ Bergen, Dept Biol, N-5020 Bergen, Norway
[2] Norwegian Univ Life Sci, Dept Chem Biotechnol & Food Sci, N-1432 As, Norway
[3] Univ Ctr Svalbard, N-9171 Longyearbyen, Norway
关键词
archaea; community analysis; 16S rRNA gene; denaturing gradient gel electrophoresis; methane; Arctic wetland;
D O I
10.1016/j.femsec.2005.01.004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Emissions of the greenhouse gas methane from Arctic wetlands have been studied extensively, though little is known about the ecology and community structure of methanogenic archaea that catalyze the methane production. As part of a project addressing microbial transformations of methane in Arctic wetlands, we studied archaeal communities in two wetlands (Solvatnet and Stuphallet) at Spitsbergen, Norway (78 degrees N) during two summer seasons. Directly extracted peat community DNA and enrichment cultures of methanogenic archaea were analyzed by nested PCR combined with denaturing gradient gel electrophoresis and subsequent sequencing of 16S rRNA gene fragments. Sequences affiliated with Methanomicrobiales, Methanobacteriaceae, Methanosaeta and Group T.3b of the uncultured crenarchaeota were detected at both sites. Sequences affiliated with Methanosarcina were recovered only from the site Solvatnet, while sequences affiliated with the euryarchaeotal clusters Rice Cluster II and Sediment I were detected only at the site Stuphallet. The phylogenetic affiliation of the recovered sequences suggested a potential of both hydrogenotrophic and acetoclastic methanogenesis at both sites. At Solvatnet, there were clear temporal trends in the archaeal community structure over the Arctic summer season. The archaeal community composition was significantly affected by factors influencing the activity of the overall bacterial community, as measured by in situ emissions Of CO2. Methane emissions at both sites were influenced more by peat temperatures and thaw depth than by the archaeal community structure. Enrichment cultures for methanogenic archaea determined that most of the methanogens detected directly in peat could grow in culture at 10 degrees C. Culture based biases were indicated in later enrichment steps by the abundant growth of a Methanosarcina strain that was not detected directly in peat samples. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:89 / 101
页数:13
相关论文
共 51 条
[41]  
ter Braak C.J. F., 1988, CANOCO REFERENCE MAN
[42]   Phylogenetic comparison of methanogen diversity in different wetland soils [J].
Utsumi, M ;
Belova, SE ;
King, GM ;
Uchiyama, H .
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2003, 49 (02) :75-83
[43]   Goose droppings as food for reindeer [J].
van der Wal, R ;
Loonen, MJJE .
CANADIAN JOURNAL OF ZOOLOGY-REVUE CANADIENNE DE ZOOLOGIE, 1998, 76 (06) :1117-1122
[44]  
von Wintzingerode F, 1997, FEMS MICROBIOL REV, V21, P213
[45]   THE EFFECT OF SOIL-MOISTURE AND THAW DEPTH ON CH4 FLUX FROM WET COASTAL TUNDRA ECOSYSTEMS ON THE NORTH SLOPE OF ALASKA [J].
VOURLITIS, GL ;
OECHEL, WC ;
HASTINGS, SJ ;
JENKINS, MA .
CHEMOSPHERE, 1993, 26 (1-4) :329-337
[46]   Microbial controls on methane fluxes from a polygonal tundra of the Lena Delta, Siberia [J].
Wagner, D ;
Kobabe, S ;
Pfeiffer, EM ;
Hubberten, HW .
PERMAFROST AND PERIGLACIAL PROCESSES, 2003, 14 (02) :173-185
[47]   Methanotrophic diversity in high arctic wetlands on the islands of svalbard (Norway) - denaturing gradient gel electrophoresis analysis of soil DNA and enrichment cultures [J].
Wartiainen, I ;
Hestnes, AG ;
Svenning, MM .
CANADIAN JOURNAL OF MICROBIOLOGY, 2003, 49 (10) :602-612
[48]   INTERANNUAL VARIATIONS IN TUNDRA METHANE EMISSION: A 4-YEAR TIME SERIES AT FIXED SITES [J].
Whalen, Stephen ;
Reeburgh, William .
GLOBAL BIOGEOCHEMICAL CYCLES, 1992, 6 (02) :139-159
[49]  
WHITMAN WB, 1992, PROKARYOTES, V2, P719
[50]   STUDIES ON DISSIMILATORY SULFATE-REDUCING BACTERIA THAT DECOMPOSE FATTY-ACIDS .3. CHARACTERIZATION OF THE FILAMENTOUS GLIDING DESULFONEMA-LIMICOLA GEN-NOV SP-NOV, AND DESULFONEMA-MAGNUM SP-NOV [J].
WIDDEL, F ;
KOHRING, GW ;
MAYER, F .
ARCHIVES OF MICROBIOLOGY, 1983, 134 (04) :286-294