Electrodeposited polytyramine as an immobilisation matrix for enzyme biosensors

被引：99

作者：

Situmorang, M ^{[1
]}

Gooding, JJ ^{[1
]}

Hibbert, DB ^{[1
]}

Barnett, D ^{[1
]}

机构：

[1] Univ New S Wales, Dept Analyt Chem, Sydney, NSW 2052, Australia

来源：

BIOSENSORS & BIOELECTRONICS | 1998年 / 13卷 / 09期

关键词：

electrodeposition; polytyramine; enzyme biosensor; covalent immobilisation; 1-ethyl-3(3-dimethylaminopropyl) carbodiimide hydrochloride; N-hydroxysuccinimide;

D O I：

10.1016/S0956-5663(98)00033-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The application of an electrodeposited polytyramine film as an immobilisation matrix for the construction of enzyme biosensors is described. Glucose oxidase (used as a model enzyme) is covalently attached to free amine groups on the polytyramine film using the coupling reagents 1-ethyl-3(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide. The resultant recognition interface consisted of multilayers of GOx immobilised onto the polymer surface. This method of constructing enzyme biosensors is shown to produce a highly reproducible and stable device. The biosensor showed no loss in electrode response after four month, of dry storage and exhibited only minor loss in response after 20 days of repeated use. The resultant biosensor had a linear range of 0.1-28 mM glucose and a detection limit of 0.01 mM. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：953 / 962

页数：10

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