The cDNA cloning and transient expression of a chicken gene encoding cytochrome P-450scc

被引：16

作者：

Nomura, O ^{[1
]}

Nakabayashi, O ^{[1
]}

Nishimori, K ^{[1
]}

Mizuno, S ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC AGR,DEPT APPL BIOL CHEM,MOL BIOL LAB,AOBA KU,SENDAI,MIYAGI 981,JAPAN

来源：

GENE | 1997年 / 185卷 / 02期

关键词：

splicing out of polyadenylation signal; conserved sequence region; transfection; enzyme assay;

D O I：

10.1016/S0378-1119(96)00645-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A cDNA clone, pcSCC1, isolated from a chicken adrenal gland library showed about 62% nucleotide sequence similarity to those of mammalian and rainbow trout P-450scc cDNAs and was proved to be the clone for chicken P-450scc by the enzymatic activity of converting 25-hydroxycholesterol to pregnenolone demonstrated in the transfected COS-7 cells. Comparison of deduced amino acid sequences shows significant conservation of heme- and steroid-binding domains but little similarity in the putative N-terminal presequence region among chicken, trout and mammals. The chicken cDNA lacks a polyadenylation signal in its 3'-UTR . Sequencing of a 3' end region of the P-450scc gene (CYP11A1) suggests that a region containing the polyadenylation signal is spliced out from the pre-mRNA.

引用

页码：217 / 222

页数：6

共 26 条

[1] RADIOIMMUNOASSAY OF PLASMA PREGNENOLONE [J].

ABRAHAM, GE ;

BUSTER, JE ;

KYLE, FW ;

CORRALES, PC ;

TELLER, RC .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1973, 37 (01) :40-45

[2]

Ausubel F.M., 1987, CURRENT PROTOCOL MOL

[3] CHARACTERIZATION OF MITOCHONDRIAL CYTOCHROMES-P-450 FROM PIG-KIDNEY AND LIVER CATALYZING 26-HYDROXYLATION OF 25-HYDROXYVITAMIN-D3 AND C27-STEROIDS [J].

BERGMAN, T ;

POSTLIND, H .

BIOCHEMICAL JOURNAL, 1991, 276 :427-432

[4] HUMAN CHOLESTEROL SIDE-CHAIN CLEAVAGE ENZYME, P450SCC - CDNA CLONING, ASSIGNMENT OF THE GENE TO CHROMOSOME-15, AND EXPRESSION IN THE PLACENTA [J].

CHUNG, B ;

MATTESON, KJ ;

VOUTILAINEN, R ;

MOHANDAS, TK ;

MILLER, WL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (23) :8962-8966

[5] AROMATASE ENZYME-ACTIVITY AND SEX DETERMINATION IN CHICKENS [J].

ELBRECHT, A ;

SMITH, RG .

SCIENCE, 1992, 255 (5043) :467-470

[6] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[7] UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING [J].

HENIKOFF, S .

GENE, 1984, 28 (03) :351-359

[8] 2 DISTINCT ENHANCERS WITH DIFFERENT CELL SPECIFICITIES COEXIST IN THE REGULATORY REGION OF POLYOMA [J].

HERBOMEL, P ;

BOURACHOT, B ;

YANIV, M .

CELL, 1984, 39 (03) :653-662

[9] HOW PROTEINS FIND MITOCHONDRIA AND INTRAMITOCHONDRIAL COMPARTMENTS [J].

HURT, EC ;

VANLOON, APGM .

TRENDS IN BIOCHEMICAL SCIENCES, 1986, 11 (05) :204-207

[10] THE RAT CLOFIBRATE-INDUCIBLE CYP4A GENE SUBFAMILY .1. COMPLETE INTRON AND EXON SEQUENCE OF THE CYP4A1 AND CYP4A2 GENES, UNIQUE EXON ORGANIZATION, AND IDENTIFICATION OF A CONSERVED 19-BP UPSTREAM ELEMENT [J].

KIMURA, S ;

HANIOKA, N ;

MATSUNAGA, E ;

GONZALEZ, FJ .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1989, 8 (07) :503-516

← 1 2 3 →