Next-generation high-density self-assembling functional protein arrays

被引：201

作者：

Ramachandran, Niroshan ^{[1
]}

Raphael, Jacob V. ^{[1
]}

Hainsworth, Eugenie ^{[1
]}

Demirkan, Gokhan ^{[1
]}

Fuentes, Manuel G. ^{[1
]}

Rolfs, Andreas ^{[1
]}

Hu, Yanhui ^{[1
]}

LaBaer, Joshua ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Harvard Inst Proteom, Cambridge, MA 02141 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 06期

关键词：

D O I：

10.1038/NMETH.1210

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.

引用

页码：535 / 538

页数：4

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