Identification of Mouse Serum miRNA Endogenous References by Global Gene Expression Profiles

被引：33

作者：

Mi, Qing-Sheng ^{[1
,2
,3
]}

Weiland, Matthew ^{[1
,2
]}

Qi, Rui-Qun ^{[1
,2
,4
]}

Gao, Xing-Hua ^{[4
]}

Poisson, Laila M. ^{[5
]}

Zhou, Li ^{[1
,2
,3
]}

机构：

[1] Henry Ford Hlth Syst, Henry Ford Immunol Program, Detroit, MI 48202 USA

[2] Henry Ford Hlth Syst, Dept Dermatol, Detroit, MI USA

[3] Henry Ford Hlth Syst, Dept Internal Med, Detroit, MI USA

[4] China Med Univ, Hosp 1, Dept Dermatol, Shenyang, Peoples R China

[5] Henry Ford Hlth Syst, Dept Publ Hlth Sci, Detroit, MI USA

来源：

PLOS ONE | 2012年 / 7卷 / 02期

关键词：

CIRCULATING MICRORNAS; PLASMA MICRORNAS; BIOMARKERS; CANCER; NORMALIZATION; MIRBASE; TARGETS; DICER; DNA;

D O I：

10.1371/journal.pone.0031278

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

MicroRNAs (miRNAs) are recently discovered small non-coding RNAs and can serve as serum biomarkers for disease diagnosis and prognoses. Lack of reliable serum miRNA endogenous references for normalization in miRNA gene expression makes single miRNA assays inaccurate. Using TaqMan (R) real-time PCR miRNA arrays with a global gene expression normalization strategy, we have analyzed serum miRNA expression profiles of 20 female mice of NOD/ShiLtJ (n = 8), NOR/LtJ (n = 6), and C57BL/6J (n = 6) at different ages and disease conditions. We identified five miRNAs, miR-146a, miR-16, miR-195, miR-30e and miR-744, to be stably expressed in all strains, which could serve as mouse serum miRNA endogenous references for single assay experiments.

引用

页数：6

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