Cyclin-dependent kinase activating kinase/Cdk7 co-localizes with PKC-ι in human glioma cells

被引：10

作者：

Bicaku, E

Patel, R

Acevedo-Duncan, M ^{[1
]}

机构：

[1] Univ S Florida, Dept Chem, Tampa, FL 33620 USA

[2] James A Haley Vet Hosp, Tampa, FL 33612 USA

来源：

TISSUE & CELL | 2005年 / 37卷 / 01期

关键词：

CAK; cdk7; PKC-iota; cell cycle; glioma; U-373MG;

D O I：

10.1016/j.tice.2004.10.004

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Cyclin-dependent kinase activating kinase (CAK) is a trimeric complex composed of cdk7, cyclin H and MAT1. CAK/cdk7 functions as a master cell cycle regulator by phosphorylating cyclin-dependent kinases for cell cycle progression. We have previously reported that protein kinase C-iota (PKC-iota) associates with CAK/cdk7. In this investigation, immunofluorescence confocal microscopy was used to provide further evidence for the co-localization of PKC-iota, with CAK/cdk7. PKC-iota was labeled with Alexa Fluor 488 (green fluorescent dye) and CAK/cdk7 was labeled with Alexa Fluor 555 (red fluorescent dye). The fusion of the red and green fluorescent colors produced a yellow color, which was used to quantify co-localization of PKC-iota and CAK/cdk7. Confocal microscopy revealed the co-localization of PKC-iota with CAK/cdk7 in both the cytoplasm and nucleus of U-373 MG cells (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：53 / 58

页数：6

共 20 条

[1] Human glioma PKC-ι, and PKC-βII phosphorylate cyclin-dependent kinase activating kinase during the cell cycle [J].