Production of cyclohexanone monooxygenase from Acinetobacter calcoaceticus for large scale Baeyer-Villiger monooxygenase reactions

被引：7

作者：

Barclay, SS

Woodley, JM

Lilly, MD

Spargo, PL

Pettman, AJ

机构：

[1] UCL, Dept Biochem Engn, London WC1E 7JE, England

[2] Pfizer Ltd, Pfizer Cent Res, Proc Res & Dev Dept, Sandwich CT13 9NJ, Kent, England

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 05期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Acinetobacter calcoaceticus; cyclohexanone monooxygenase; fermentation;

D O I：

10.1023/A:1005610815727

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cyclohexanone monooxygenase was produced from Acinetobacter calcoaceticus grown on a medium containing both glutamate (30 g l(-1)) and cyclohexanol (1 g l(-1)). Productivity was increased to 650 U l(-1), an order of magnitude greater than previous production methods, thereby enhancing the potential commercial utility of this enzyme.

引用

页码：385 / 388

页数：4

共 7 条

[1] Comparison of microbiologically and enzymatically mediated Baeyer-Villiger oxidations: Synthesis of optically active caprolactones [J].