An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas' disease

被引：95

作者：

Assumpcao, Teresa C. F. ^{[2
]}

Francischetti, Ivo M. B. ^{[1
]}

Andersen, John F. ^{[1
]}

Schwarz, Alexandra ^{[3
]}

Santana, Jaime M. ^{[2
]}

Ribeiro, Jose M. C. ^{[1
]}

机构：

[1] NIAID, Vector Biol Sect, Lab Malaria & Vector Res, NIH, Rockville, MD 20852 USA

[2] Univ Brasilia, Lab Host Parasite Interface, BR-70910900 Brasilia, DF, Brazil

[3] Ruhr Univ Bochum, Dept Special Zool, D-44780 Bochum, Germany

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2008年 / 38卷 / 02期

基金：

美国国家卫生研究院;

关键词：

hematophagy; saliva; transcriptome; salivary gland; Triatoma infestans; feeding; sialome;

D O I：

10.1016/j.ibmb.2007.11.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Triatoma infestans is a hemiptera, vector of Chagas' disease that feeds exclusively on vertebrate blood in all life stages. Hematophagous insects' salivary glands (SG) produce potent pharmacological compounds that counteract host hemostasis, including anticlotting, antiplatelet, and vasodilatory molecules. To obtain a further insight into the salivary biochemical and pharmacological complexity of this insect, a cDNA library from its SG was randomly sequenced. Also, salivary proteins were submitted to two-dimensional gel (2D-gel) electrophoresis followed by MS analysis. We present the analysis of a set of 1534 (SG) cDNA sequences, 645 of which coded for proteins of a putative secretory nature. Most salivary proteins described as lipocalins matched peptide sequences obtained from proteomic results. Published by Elsevier Ltd.

引用

页码：213 / 232

页数：20

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